Membrane-bound cytochrome P-450 mimic. Polymerized vesicles as microreactors
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چکیده
منابع مشابه
Membrane-bound cytochrome P-450 mimic. Polymerized vesicles as microreactors.
Supplementary Material Available: Preparative methods for the starting materials, stereochemical assignments of 1,3-diols listed in Table I, and 400-M Hz 'H N M R spectra of acetonides of 1-3 and 7 (6 pages). Ordering information is given on any current masthead page. The Cytochrome P-450 dependent monooxygenases are mem brane-bound enzymes that catalyze a great variety of reactions, among whi...
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Purified rat liver microsomal cytochrome P-450 and NADPH-cytochrome P-450 reductase were co-reconstituted in phosphatidylcholine-phosphatidylethanolamine-phosphatidylserine vesicles using a cholate dialysis technique. The co-reconstitution of the enzymes was demonstrated in proteoliposomes fractionated by centrifugation in a glycerol gradient. The proteoliposomes catalyzed the N-demethylation o...
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1. The topography of cytochrome P-450 in vesicles from smooth endoplasmic reticulum of rat liver has been examined. Approx. 50% of the cytochrome is directly accessible to the action of trypsin in intact vesicles whereas the remainder is inaccessible and partitioned between luminal-facing or phospholipid-embedded loci. Analysis by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis revea...
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The presence of a very active cytochrome P-450-dependent drug-metabolizing system in the olfactory epithelium has been confirmed by using 7-ethoxycoumarin, 7-ethoxyresorufin, hexobarbitone and aniline as substrates, and the reasons for the marked activity of the cytochrome P-450 in this tissue have been investigated. The spectral interaction of hexobarbitone and aniline with hepatic and olfacto...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 1986
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja00279a098